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Introduction In vitro synthetic biology has received conside
2019-12-16

Introduction In vitro synthetic biology has received considerably less attention than in vivo processes so far (Foster and Church, 2007). However, cell-free biosynthetic production is very likely to become important for many biotechnological challenges for reasons such as (energy) efficiency, syste
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br Experimental section br Author contributions br
2019-12-16

Experimental section Author contributions Acknowledgements Introduction Many (-)-p-Bromotetramisole Oxalate in cells are sequential enzymatic reactions where the product of one enzyme serves as the substrate of a second enzyme (1, 2). Experimental evidence suggests that some of these co
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Acknowledgments The authors thank Dr Vladimir Poltoratsky an
2019-12-14

Acknowledgments The authors thank Dr. Vladimir Poltoratsky and Zhihui Xiao for their help with the in vitro FRET assay. Support for this research was provided by Saint John’s University. Introduction We predicted the existence of endothelium-derived contractile factors (EDCFs) in 1982 [1]. In 1
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Cyclin D inhibits the transcriptional activity of the transc
2019-12-14

Cyclin D1 inhibits the transcriptional activity of the transcription factors myogenin and MEF2 [39,40]. In addition, myogenin activates MEF2 expression, which feeds back to amplify the myogenin promoter [38]. In this study, DGKδ suppression, in addition to the increase of cyclin D1, inhibited myogen
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DNA damage represents a persistent threat
2019-12-13

DNA damage represents a persistent threat to genomic stability. A critical link exists between DNA mutation, chromosomal rearrangement and cancer development. In myeloid malignancies, various chromosomal translocations and/or mutations increased cellular reactive oxygen species (ROS), followed by DS
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br DGAT protein interactions Although DGAT and DGAT are memb
2019-12-13

DGAT-protein interactions Although DGAT1 and DGAT2 are membrane proteins of the ER, the ER contains within its extensive three-dimensional network different, unique structural and functional protein domains that arise from the non-uniformity imposed on it by the intracellular structures it intera
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Introduction Klebsiella pneumoniae a Gram negative enteric b
2019-12-13

Introduction Klebsiella pneumoniae, a Gram-negative enteric bacterium, is an organism with significance in both biomedicine and biotechnology. Some of the strains of K. pneumoniae are opportunistic pathogens, which can cause suppurative infection, pneumoniae and urinary tract infection in humans (B
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This investigation est http www apexbt com media
2019-12-13

This investigation establishes that there is a genetic difference in the enolase loci that correlates with both vegetative growth and virulence phenotypes. DNA sequence analysis of enolase loci from seven P. larvae strains reveals a single SNP that alters the protein sequence at position 331 (Table
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Despite the fact that the precise relevance
2019-12-13

Despite the fact that the precise relevance of Ube2E self-ubiquitination in vivo is still elusive, findings of Sarkari et al. do establish Ube2E1 ubiquitination in Aliskiren [36]. In vivo self-ubiquitination of Ube2E1 is further supported by the high-throughput study aimed to identify all ubiquitin
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Real time PCR as described previously with minor
2019-12-13

Real-time PCR, as described previously with minor modifications, was used to detect DNA . The PCR mixture (Qiagen) was as follows: 5mM MgCl2, 2.5μl of 10x PCR Buffer, 0.125μl of HotStarTaq DNA Polymerase, 0.25mM of each dNTP, 0.4μM of each primer and 0.1μM of FAMlabelled probe (‘5-aacaattggagggcaagg
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According to this theory the chemical potential
2019-12-13

According to this theory, the chemical potential of the basic hydrate in the equilibrium condition can be calculated as: , and denote the chemical potentials of the basic hydrate, water and gas, respectively. Also, is the number of gas molecules per water molecules in the basic hydrate. After su
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MB05032 mg No orthologs could be found in
2019-12-13

No orthologs could be found in our B. oleracea BAC library for AtCk1/2, which could mean that it is unique to Arabidopsis, or that we failed to detect it in our library. However, the second possibility is unlikely considering that Southern blotting analysis with four restriction enzymes disclosed fr
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br Materials and methods br Results
2019-12-13

Materials and methods Results Discussion In particular, we showed that pancreatic neuroendocrine tumor Solasodine recognize cAMP as a stimulatory signal for cell proliferation. Conversely, cAMP was found to have an inhibitory effect on cell proliferation in bronchial carcinoids cells. The d
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br Signaling of DDR receptor and its regulation Being
2019-12-12

Signaling of DDR2 receptor and its regulation Being the tyrosine kinase receptor, downstream signaling of DDR2 receptor is initiated by phosphorylation of cytoplasmic tyrosine residue upon ligand binding (Type II collagen) but the authentic facts or detail information of tyrosine phosphorylation
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br Conflict of interest br
2019-12-12

Conflict of interest Acknowledgments We thank Jianru Zuo (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China) for providing fbr11-1 seeds. We are grateful to Carol MacKintosh and the DSTT at the University of Dundee (UK) for anti-RD21 antibodies. This
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